The reagents described below
were produced in our lab and are available for research purposes.
|
pBlue3'LTR-luc |
| Characteristics |
HIV-1 LTR--luciferase
reporter construct with the complete promoter element of subtype
B isolate LAI. Used to produce LTR
constructs for HIV-1 subtypes A, B, C, D, E, F, and G. The
LTR can be activated by the Tat protein.
Contains a 1426 bp BglI-XhoI fragment from
pBluescript KS (+) with the ColE1 origin, a 719 bp XhoI-HindIII
LAI 3' LTR fragment, a 1951 bp HindIII-BamHI pGL3 luciferase
gene, and a 1625 bp BamHI-BglI fragment derived from pSV2CAT,
which encompasses an SV40 polyA site and a pBluescript KS
(+) fragment. Construct size: 5720 bp. |
| Reference |
Jeeninga R, Hoogenkamp
M, Armand-Ugon M, de Baar M, Verhoef K and Berkhout B. 2000.
Functional differences between the LTR transcriptional promoters
of HIV-1 subtypes A through G. Journal
of Virology 74, 3740-3751. |
| Availability |
Requests should be
addressed to the NIH AIDS
reagents repository, catalog no. 4788. Alternatively, this
reagent can be obtained through the NIBSC
centralized facility for AIDS reagents. |
|
|
|
LTR Exchange Reporter
Clones |
| Characteristics |
The LTR region (nt
-147 to +63 relative to the transcription start site) from a
subtype A, C, D, E, F, or G patient isolate was PCR-amplified
and exchanged into pBlue3'LTR-luc in the place of the LAI LTR.
The exchange region contains two NF-kB
enhancers, three Sp1 binding sites, the TATAA box, and the TAR
hairpin motif. Construct size: 5720
bp. |
| Reference |
Jeeninga R, Hoogenkamp
M, Armand-Ugon M, de Baar M, Verhoef K and Berkhout B. 2000.
Functional differences between the LTR transcriptional promoters
of HIV-1 subtypes A through G. Journal
of Virology 74, 3740-3751. |
| Availability |
Requests should be
addressed to the NIH AIDS
reagents repository, catalog no. 4788. Alternatively, this
reagent can be obtained through the NIBSC
centralized facility for AIDS reagents. |
|
|
On a collaborative basis we can provide several
other reagents, including:
Set of LAI molecular clones
with subtype-specific LTRs: subtype B molecular clone LAI with
the enhancer/promoter region of the other subtypes. Journal
of Virology 74, 3740-3751.
Doxycycline-driven HIV-1: subtype B molecular
clone LAI containing the elements of the Tetracycline system. Replication
is strictly dependent on the tetracycline analogue doxycycline.
(Verhoef K, Marzio G, Hillen
W, Bujard H and Berkhout B. 2001. Strict control of HIV-1 replication
by a genetic switch: Tet for Tat. Journal
of Virology, 75, 979-87. Several new and improved versions of
this conditional replicating virus are currently available and details
can be provided at request.
|
